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Planta Med. Jan;71(1) Expression of Vitreoscilla hemoglobin enhances growth of Hyoscyamus muticus hairy root cultures. Wilhelmson A(1), Kallio. Plant Cell Rep. Mar;13(6) doi: /BF An unusual root tip formation in hairy root culture of Hyoscyamus muticus. Jaziri M(1), Homes . Hairy root were induced by inoculation of Agrobacterium rhizogenes in sterile seedlings of Datura stramonium and Hyoscyamus niger. The transformed roots.

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You can login by using one muticjs your existing accounts. Remember me on this computer. Help Center Find new research papers in: Although genetic and metabolic stability has often been connected to transgenic hairy roots, there are only few reports on how a very long-term subculturing effects on the production capacity of hairy roots. Cryopreservation Biotechnology relies to a great part on working with cell cultures and continues to offer a future beyond depletion of natural resources.

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Overexpression of one enzyme obtained from the Second Military Medical University Shang- often renders subsequent reactions more rate-limiting, and thus hai, China and germinated into plants. Enhancement of scopolamine production in Hyoscyamus muticus L. Finally they can be detached and grown mutocus independent hairy root clones.

Plant cell haity can be divided in two main classes, differentiated and undifferentiated cell cultures. Recent efforts have been aimed at h6h, both driven by the CaMV 35S promoter and the nptII increasing the flux through the biosynthetic pathways.

It is concluded that, as in the case of transgenics that include The current study provides an effective approach for commer- both pmt and h6h in H. No use, distribution or reproduction is permitted which does not comply with these terms. Basically, cryopreservation methods are divided in three categories, those based on slow freezing of the muticuus by using temperature gradients Even though successful cryopreservation methods for a number of plant species have been established, a considerable experimentation is always needed to optimize all the steps in cryopreservation procedure Reed, Tropane alkaloid production in root cultures of Duboisia myoporoides obtained by repeated selection.


Establishment of hairy root cultures of Datura stramonium. For a number of desired plants, hairy roots have been induced for the commercial scale production of metabolites, often yielding even higher amounts of metabolites than that of the muticue plant Jouhikainen et al.

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An unusual root tip formation in hairy root culture of Hyoscyamus muticus.

Hairy roots were cultivated for 14 days on solid medium. It is interesting to note that the highest similarity in protein level was observed with B. Engineering tropane biosynthetic pathway in Hyoscyamus niger hairy root cultures.

To our knowledge, this is the actions on the central nervous system, there is currently a fold highest scopolamine content achieved through genetic engineer- higher commercial demand for scopolamine, in the N- ing of a plant. Cryopreservation method for hairy roots of Hyoscyamus muticus was developed to replace laborious subculturing, and although the post-thaw recovery rates remained low, the expression of transgene remained unaltered in cryopreserved roots.

R Foundation for Statistical Computing.

Cryopreservation Several cryopreservation methods were tested for long-term storage of H. SH performed most of the experiments, evaluated the data and designed and wrote the manuscript together with K-MO-C. In Table 1the accumulation of tropane alkaloids and growth during the year follow-up is presented. Root tips were transferred into cryo-vials and incubated at room temperature together with cryoprotectant for 1 h.

Effect of salicylic acid, methyl jasmonate, ethephon and cantharidin hyoscyzmus anthraquinone production by Rubia cordifolia callus cultures transformed with the rolB and rolC genes.

Cryoprotection on the other hand covers the use of permeable agents which function through a colligative effect and usually result in the increase in membrane fluidity. The Hair thank Professor T. Citations Publications citing this paper.

Agrobacterium Cu,ture is a soil bacterium, which is able to deliver a part of its own plasmid-DNA T-DNA into the nuclear genome of the plant cell. The hairy root clone KB7 or formerly 13A7 of H. Genetic stability of hairy root cultures of Datura stramonium. In total, 41 P, 50 H, and see Fig.

Expression of Vitreoscilla hemoglobin enhances growth of Hyoscyamus muticus hairy root cultures.

Somaclonal variation in transformed roots and protoplast-derived hairy root clones of Hyoscyamus muticus. However, cryopreservation of plant cells still mainly relies on the fully empirical testing, which makes the method development very laborious.


Decades of hakry work has turned this hairy root muticuus into a valuable biotechnological application Georgiev et al. The sequence of H. Thawing was performed using four different procedures, displayed in Figure 1.

Northern blot analysis showed that line hancement of scopolamine accumulation in cultured hairy root H11 had a relatively higher h6h transcript level than T3 and T10, lines. The hy- few genera of the plant family Solanaceae, including Hyoscyamus, droxylase gene from Hyoscyamus niger has been introduced into Duboisia, Atropa, and Scopolia, are able to produce biologically Atropa belladonna, a typical hyoscyamine-rich tropane alkaloid- active nicotine and tropane alkaloids simultaneously 1—3.

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Cryopreservation of horseradish hairy root cultuee by encapsulation-dehydration. Validation of cryopreservation protocols for plant germplasm conservation: This phenomenon is commonly seen with plant cell and tissue culture cryopreservation resulting in step to step empirical testing for discovery of putative suitable cryostorage method.

Cryopreservation of transformed hairy roots of Artemisia annua. The first analyses were performed using immunoassays while later the alkaloids were analyzed by GC-MS. Root tips were thawed in a following way unless otherwise described. B Time courses of growth of five randomly selected hairy root lines.

Showing of 48 extracted citations. Flow cytometric investigations of diploid and tetraploid plants and in vitro cultures of Datura stramonium and Hyoscyamus niger.

Genetic stability of hairy roots is well recognized. Molecular farming in tobacco hairy roots by triggering the secretion of a pharmaceutical antibody. All lines expressing h6h gene overexpression in A. Production of tropane alkaloids in diploid and tetraploid plants and in vitro hairy root cultures of Egyptian hary Hyoscyamus muticus L.